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KMID : 0380019980130010090
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Korean Journal of Biotechnology and Bioengineering
1998 Volume.13 No. 1 p.90 ~ p.95
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Process Development of Concentration of n-3 PUFAs from Fish Oil by Means of Lipase
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Jin Young-Suh
Hur Byung-Ki
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Abstract
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Experiments on the process development for the concentration of polyunsaturated fatty acid (PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil by using acyl chain specificity of Candida cylindracea lipase were performed. Five kinds of oils were hydrolyzed with Candida cylindracea lipase. Among then, Candida cylindracea lipase just had low activity on the PUFAs-rich fish oil. After the hydrolysis of fish oil, free fatty acid was removed and fatty acid components of glyceride mixtures were analyzed. When the hydrolysis was about 70%, the DHA content in the glyceride mixture was about three times more than that in the original fish oil. The EPA and stearidonic acid contents in the glyceride mixtures, however, were similar to that of the original fish oil. In this work, these results showed that the concentration process of PUFAs by using the acyl chain specificity of Candida cylindreacea lipase was effective in producing glycerides that contained a high concentration of PUFAs in good yield.
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KEYWORD
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Candida cylindracea lipase, acyl chain speicificity, polyunsaturated fatty acids(PUFAs), docosahexaenoic acid(DHA), eicosapentaenoic acid(EPA)
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